Slicing a roach on a microtome, to stain and view under a microscope

Jul 2, 2024 6:35 PM

aloofloofah

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Slicing a roach on a microtome, to stain and view under a microscope

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I really like the scientific labeling of the specimens. "ROACH 1" and "ROACH 2".

1 year ago | Likes 2 Dislikes 0

1 year ago | Likes 3 Dislikes 0

Nicely hidden as usual toolgifs

1 year ago | Likes 3 Dislikes 0

"It's only wafer-thin."

1 year ago | Likes 13 Dislikes 0

This is the comment I came here to read.

1 year ago | Likes 4 Dislikes 0

Cockroach carpaccio!

1 year ago | Likes 3 Dislikes 0

Forbidden bonito flakes

1 year ago | Likes 4 Dislikes 0

coldcuts of the future!

1 year ago | Likes 22 Dislikes 0

But sir, it is only waaffer thiiin

1 year ago | Likes 3 Dislikes 0

Very neat, but also...

1 year ago | Likes 13 Dislikes 2

that's one sharp blade

1 year ago | Likes 3 Dislikes 0

Next time I'm at the deli and they ask me how I want the meat sliced, I'll show them this.

1 year ago | Likes 3 Dislikes 0

Is that transparent thing starch or epoxy? If it can be done with starch then I'd say you're about to invent a novelty food

1 year ago | Likes 1 Dislikes 0

it's paraffin wax. the samples are usually fixed in formalin, then dehydrated with alcohol, cleared with xylene, and infiltrated with paraffin, before being embedded in a wax block to be cut on the microtome

1 year ago | Likes 2 Dislikes 0

Paraffin is edible or at least non-toxic, right? *starts planning business idea*

1 year ago | Likes 2 Dislikes 0

This person is doing an outstanding job. They’ve sliced at least one or two roaches before this.

1 year ago | Likes 5 Dislikes 0

Naughty roaches go in the r o a c h s l i c e r

1 year ago | Likes 54 Dislikes 1

Death of a thousand cuts

1 year ago | Likes 5 Dislikes 0

I've done this before! Except with mouse parts. I worked in a reproductive genetics lab in college. Did some cool stuff.

1 year ago | Likes 3 Dislikes 0

That's how thin I want my prosciutto

1 year ago | Likes 4 Dislikes 0

rochsciutto

1 year ago | Likes 3 Dislikes 0

Like the scene from "the cell"

1 year ago | Likes 4 Dislikes 0

h o r s e

1 year ago | Likes 3 Dislikes 0

That reminds me of that horse scene from "The Cell"

1 year ago | Likes 18 Dislikes 0

Why was it still moving though? That detail is what messed me up the most.

1 year ago | Likes 2 Dislikes 0

I wish I knew. I just happen to walk into the room while my parents were watching that scene and I went "Nope" and walked back out.

1 year ago | Likes 2 Dislikes 0

Not the first rodeo

1 year ago | Likes 6 Dislikes 1

Was the last for the roach though

1 year ago | Likes 1 Dislikes 0

At first I was like "yeah yeah I know this process." Absolutely not. Absolutely awesome!

1 year ago | Likes 3 Dislikes 0

1 year ago | Likes 23 Dislikes 0

Damn they're good

1 year ago | Likes 3 Dislikes 0

Sneaky fucker

1 year ago | Likes 7 Dislikes 0

Pass. I remember a a kid seeing that scene of the house being split and spread apart by panes of glass on the movie, The Cell, and that fucked me up

1 year ago | Likes 4 Dislikes 0

Horse*, not house

1 year ago | Likes 1 Dislikes 0

When you said "the house" I thought you meant like in one of those "amazing cross-sections" things and was wondering why that would be any disturbing… yeah I understand the typo now :/

1 year ago | Likes 2 Dislikes 0

Oh yeah, sorry. Horse*

1 year ago | Likes 2 Dislikes 0

Roach sashimi, for science

1 year ago | Likes 312 Dislikes 1

With that marbling it's gotta be wagyu roach..

1 year ago | Likes 3 Dislikes 0

😂🤣

1 year ago | Likes 1 Dislikes 0

Oh I've got time for sushis and sashimis

1 year ago | Likes 15 Dislikes 0

Ah..the old Hexapoda vs. Crustacea decision at a fine restaurant. Bug or sea bug.

1 year ago | Likes 8 Dislikes 0

1 year ago | Likes 2 Dislikes 0

It's roach carpaccio.

1 year ago | Likes 3 Dislikes 0

Interesting, this is actually what I do for a living. The only difference is I do frozen sections for skin cancer removal, but there was recent discussion about having me do paraffin wax sections like this for dermatopathology. Cool to see.

1 year ago | Likes 8 Dislikes 0

"Cool to see." Yeah, that's why you're at that job and I'm not.

1 year ago | Likes 1 Dislikes 0

Skin is a pain in the ass tbh outside of frozens. Did dermpath histo for like two years before switching.

1 year ago | Likes 1 Dislikes 0

Yea it should be interesting. Getting the edges down can be annoying and I imagine it is probably harder to do in wax. As it is I have lots of free time between frozen sections and could be making more my money doing both.

1 year ago | Likes 1 Dislikes 0

It's like an MRI but not

1 year ago | Likes 8 Dislikes 0

Please nobody tell OP about "cat scans" :(

1 year ago | Likes 1 Dislikes 0

An MR-die

1 year ago | Likes 5 Dislikes 0

If you don't care whether the subject survives the scan or not

1 year ago | Likes 2 Dislikes 0

Mechanical Roach Incisor

1 year ago | Likes 5 Dislikes 0

FOUND THE LOGO! (hint in response) It seems like the first thing I have to do when I see the tag. Then I can go back and really watch it.

1 year ago | Likes 97 Dislikes 3

I just watched the whole thing, next day I realized it was from Tool Gifs and I had to re-watch the whole thing to find it. Damn thing was really well hidden this time!

1 year ago | Likes 1 Dislikes 0

I almost lost my goddamn mind looking for it.

1 year ago | Likes 5 Dislikes 1

bubbles!

1 year ago | Likes 3 Dislikes 0

Hint: just a little half way through

1 year ago | Likes 30 Dislikes 1

Answer: in the bubbles on the bottom of the glass dish

1 year ago | Likes 24 Dislikes 0

i would never have seen that without the direct answer. I had to look 3 times even with that before i found it

1 year ago | Likes 8 Dislikes 0

Got to be honest I thought y'all were trying to get the brand logo of the glass dish. I was like, y'all that interested in pyrex? Then I saw it.. cool gifs indeed

1 year ago | Likes 2 Dislikes 0

Ffs I found it too. Camera is too shaky but the spot and job was excellent.

1 year ago | Likes 5 Dislikes 0

This kills the roach...

1 year ago | Likes 475 Dislikes 3

...for now.

1 year ago | Likes 1 Dislikes 0

Maybe this is how we could find out what's wrong with greedy liars.

1 year ago | Likes 1 Dislikes 0

the roach is a paid actor

1 year ago | Likes 1 Dislikes 0

Most expensive way to kill a cockroach ever

1 year ago | Likes 16 Dislikes 0

He isn't dead first!
They slice them alive!?!?!?!?

1 year ago | Likes 9 Dislikes 3

Tis but a flesh wound.

1 year ago | Likes 2 Dislikes 0

Betcha that fucker still survived

1 year ago | Likes 1 Dislikes 0

No, they'll glue him back together

1 year ago | Likes 35 Dislikes 0

And rehydrate him! The ethyl acetate is just temporary….

1 year ago | Likes 11 Dislikes 0

What do they place the bug into for slicing? Is it frozen?

1 year ago | Likes 1 Dislikes 0

but the glory is eternal

1 year ago | Likes 8 Dislikes 0

As are the pictures. People.

1 year ago | Likes 2 Dislikes 0

1 year ago | Likes 142 Dislikes 0

I've been watching this for ten minutes and Mickey hasn't given himself or Donald a single slice. That's some true love for man's best friend right there. 💕💓💕 Hang in there little buddy. When the bread runs out I have a feeling you're gonna be feasting on roasted duck.

1 year ago | Likes 7 Dislikes 0

Out of all mickey mouse moments this one always stayed in my mind

1 year ago | Likes 20 Dislikes 0

Seems like typical Disney sharing their wealth

1 year ago | Likes 9 Dislikes 0

Hey this is what I do for a living! (Except I cut people parts, not roaches)

1 year ago | Likes 25 Dislikes 0

Normally when I say I’m a Histologist, people ask what I love about History.

1 year ago | Likes 2 Dislikes 0

I want this done to my brain after I die

1 year ago | Likes 4 Dislikes 0

People are the canvas of my art... That's how i describe my work. So many pretty colours

1 year ago | Likes 4 Dislikes 0

Mohs'? That's what I do.

1 year ago | Likes 4 Dislikes 0

I don’t miss doing Moh’s. Way too stressful haha

1 year ago | Likes 1 Dislikes 0

That's one aspect of my work too, in the UK

1 year ago | Likes 2 Dislikes 0

Me too! I love seeing my job on sites like this, because most people have no idea it exists.

1 year ago | Likes 8 Dislikes 0

I can’t believe I’m seeing histology in public, I assumed only Path’s knew we existed.

1 year ago | Likes 1 Dislikes 0

Mm deli meats

1 year ago | Likes 1 Dislikes 0

Okay I've seen some cool shit before, but this is REALLY cool.

1 year ago | Likes 755 Dislikes 1

Yeah yeah, that's super neat and everything, but where are the roach paper sticky notes you promised me, op?

1 year ago | Likes 1 Dislikes 0

But can they see why kids like cinnamon toast crunch?

1 year ago | Likes 1 Dislikes 0

I'm a scientist who literally spends all week either doing science shows on stage, or teaching science to groups, and a lot of my time around that is trying to find mind blowing things in science I can direct others onto if they're fascinated. I spend hours a week trying to find amazing and cool and mindblowing shit.

This is REALLY fucking cool.

1 year ago | Likes 9 Dislikes 0

You talking about the roach butthole you can see?

1 year ago | Likes 1 Dislikes 0

I was legitimately jaw dropped as I was soaking this in. That is BALLER!

1 year ago | Likes 6 Dislikes 0

Ill take a pound of swiss, a pound of turkey and a half pound of roach sliced thin enough to observe under a microscope please!

1 year ago | Likes 4 Dislikes 0

How the detect cancer and such with biopsy.

1 year ago | Likes 1 Dislikes 0

Science Imgur go brrrrrrrrrrrrt

1 year ago | Likes 10 Dislikes 0

Not for the roaches!

1 year ago | Likes 1 Dislikes 0

I'll donate my body to science once I'm dead, if they do this with it.

1 year ago | Likes 1 Dislikes 0

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[deleted]

1 year ago (deleted Nov 6, 2024 12:21 PM) | Likes 0 Dislikes 0

1 year ago | Likes 1 Dislikes 0

Cooler. Nice.

1 year ago | Likes 3 Dislikes 0

I've worked with cryotomes. Getting slices to not fold in on themselves and actually stay on the glass slide nearly broke me on several occasions.

1 year ago | Likes 13 Dislikes 0

I've found getting them to stay on the slide to be the easiest part, getting the bastard things to not fold over though, that's an art in itself

1 year ago | Likes 2 Dislikes 0

Meh, depends on the tissue. Uterus, lungs, and non-fatty lymph nodes are cake. Anything with fat like sentinel nodes or skin are a bitch.

1 year ago | Likes 2 Dislikes 0

I usually work with brain tissue in the cryostats

1 year ago | Likes 2 Dislikes 0

Also makes sense why folding is an issue with how fatty brains are.

1 year ago | Likes 2 Dislikes 0

yup. if they were less fatty I'd be looking at FFPE instead, but we play the hand we're dealt etc.
getting them to adhere at least is easy enough, though making the gelatin-coated slides is time consuming

1 year ago | Likes 2 Dislikes 0

The Visible Human Project.
https://youtu.be/ojCNUoVfzh4?si=_Iks__Kq1zWMj5Gz

1 year ago | Likes 4 Dislikes 0

Go into pathology. We do this every day but with human tissues.

1 year ago | Likes 1 Dislikes 0

It's why I wanted to be a pathologist

1 year ago | Likes 3 Dislikes 0

I genuinely love how many people love this. I do a lot of this type of stuff for my research in oncology, it’s easy to forget how neat the process is. It’s largely called « histology » for those curious.

1 year ago | Likes 4 Dislikes 0

I genuinely didn’t think anyone outside of Pathology knew we histologists existed haha

1 year ago | Likes 1 Dislikes 0

Right?! I’m genuinely floored by the interest in this, but absolutely love it! Hi fellow histologist!

1 year ago | Likes 2 Dislikes 0

Heya! Yeah my jaw hit the floor when I saw a cassette on Imgur with paraffin haha

1 year ago | Likes 1 Dislikes 0

The roach is going to be OK, right... right?

1 year ago | Likes 565 Dislikes 7

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1 year ago (deleted Nov 6, 2024 12:21 PM) | Likes 0 Dislikes 0

Turned it into 2D.

1 year ago | Likes 3 Dislikes 0

...yes

1 year ago | Likes 2 Dislikes 0

10 years with the same joke, probably more, it's beating a dead horse at this point

1 year ago | Likes 1 Dislikes 1

1 year ago | Likes 1 Dislikes 1

Oh for sure, you just gotta stack the layers back together - easy peasy

1 year ago | Likes 24 Dislikes 0

The roach is definitely no less alive after this process than it was before it.

1 year ago | Likes 16 Dislikes 0

v

1 year ago | Likes 9 Dislikes 0

Yeah! it lives on a farm up north.

1 year ago | Likes 9 Dislikes 0

v

1 year ago | Likes 21 Dislikes 0

1 year ago | Likes 8 Dislikes 0

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1 year ago | Likes 1 Dislikes 0

1 year ago | Likes 1 Dislikes 0

They can survive nuclear radiation, lil bro can take this no sweat

1 year ago | Likes 8 Dislikes 0

Yeah, it made a full recovery dawg

1 year ago | Likes 4 Dislikes 0

I came to the comments first because I'm afraid to watch...

1 year ago | Likes 4 Dislikes 0

1 year ago | Likes 4 Dislikes 0

This kills the roach.

1 year ago | Likes 113 Dislikes 0

Lies

1 year ago | Likes 11 Dislikes 0

We finally found something that does

1 year ago | Likes 1 Dislikes 0

Nahh, ‘‘tis but a scratch….just a flesh wound…”

1 year ago | Likes 2 Dislikes 0

Pretty sure they killed the roach first, then sliced it up.

1 year ago | Likes 1 Dislikes 0

Karl!

1 year ago | Likes 1 Dislikes 0

Lies make baby Jesus cry

1 year ago | Likes 4 Dislikes 0

Too good

1 year ago | Likes 2 Dislikes 0